In the film, Pinocchio was first introduced as a lifeless puppet. When it was time for bed, Geppetto caught sight of a wishing star and wished Pinocchio to become a real boy. Once Geppetto fell asleep, his home was visited by the Blue Fairy, who brought Pinocchio to life and Jiminy Cricket became his official conscience to tell him right from wrong, for, if Pinocchio proved himself brave, truthful and unselfish, he would be transformed into a real boy. Geppetto discovered his puppet is alive and celebrated along with Figaro the kitten and Cleo the goldfish. As of that night, Pinocchio's journey to boyhood has begun.
The next morning, Pinocchio is ready for school, but is stopped by a pair of shady con artists: a sly fox named J. Worthington Foulfellow (a.k.a. "Honest John") and his mute minion, Gideon, who trick him into working for Stromboli, a puppeteer. They tell him being rich and famous is the only way to live. Pinocchio listens, believes, and ends up following the wrong path. Jiminy tries to stop him but is unsuccessful, so he chases after him.
Pinocchio immediately becomes the star of Stromboli's marionette show. Stromboli is paid beyond his wildest dreams for Pinocchio's magnificent performance. After the show, Pinocchio and Stromboli are dining and the man's true nature is revealed as parsimonious, evil and rotten. He cages Pinocchio and threatens him to perform. Pinocchio tries to tell the Blue Fairy the truth, but he doesn't know how. Pinocchio manages to escape or flee with the help of Jiminy and the Blue Fairy.
During their trip home, Pinocchio and Jiminy race home, but Pinocchio is stopped once again by Foulfellow and Gideon. They tell Pinocchio he's sick and the only cure is a vacation on Pleasure Island. They take him to the Coachman, along with many other boys, including a brat named Lampwick, who Pinocchio calls his best friend.
Once the place is torn apart, everyone has vanished, except Lampwick and Pinocchio, who are smoking and drinking while playing pool. Once Jiminy confronts the two, he is so upset, he storms out. Soon, Jiminy discovers the plan; Pleasure Island has the terrifying power to transform bad boys into donkeys, which the Coachman sells into slavery, and rushes back to get Pinocchio. Lampwick's hideous transformation is complete, but Pinocchio and Jiminy escape the island. Unfortunately, Pinocchio has grown donkey ears and a tail.
The two reach home but realize there's no one home. They sit and wait on the steps for everyone to return. Suddenly, the Blue Fairy comes in the shape of a dove and gives them a letter which tells them Geppetto was eaten by Monstro the Whale.
The pair starts searching the ocean for Monstro with very little luck. When they ask sea creatures such as clams and seahorses, they swim and hide in fear at the mention of Monstro's name. Meanwhile, after a nap, Monstro awakens and begins an eating frenzy. Everything in his path was either devoured or destroyed (including Pinocchio). Once Monstro was found, Pinocchio was able to reunite with Geppetto, Figaro, and Cleo. Pinocchio soon thinks of a plan to escape Monstro by making him sneeze.
Once Pinocchio is able to get Monstro to sneeze, the enraged animal chases after him and his father. The whale destroys the raft, sending Pinocchio and Geppetto into the unforgiving sea. After witnessing his father almost drowning, Pinocchio grabs him and swims to shore as quick as he can, but it's too late. Even before he gets there, Monstro slams into a rocky wall, creating a forty foot tidal wave. Gepetto, Figaro, Cleo, and Jiminy survive. When Jiminy looks for Pinocchio, he makes a very depressing discovery. Pinocchio is lying face down in a large puddle, in which he has drowned.
Geppetto, Figaro, Cleo, and Jiminy return home and mourn the loss of Pinocchio. Then the Blue Fairy revives Pinocchio and transforms him into a real human boy because he has now proved himself brave, truthful and unselfish, much to the joy of the others. Jiminy is then awarded a certified 14-karat conscience badge.
- He was voiced by the late Dickie Jones in his debut.
- He had a big part in Avengers: Age of Ultron since Ultron was somehow his Marvel counterpart and a dark parallel of Pinocchio.
- He is mentioned in the Descendants Official Mobile Game when a student named Mikey said, "We where, Pinocchio play poker, bad".